Gel permeation chromatographic method for drug protein binding studies
نویسندگان
چکیده
منابع مشابه
A gel filtration method for studies on protein iron-binding.
This communication reports the use of a gel filtration technique and radioactive iron for rapid determination of protein-iron binding in complex mixtures. Many methods have been used to investigate the characteristics of iron binding by mammalian serum. Calorimetric techniques have been used to measure the iron-binding capacities of whole seral. Electrophoretic2y3 and precipitation41s methods, ...
متن کاملImmobilization of alpha1-acid glycoprotein for chromatographic studies of drug-protein binding II. correction for errors in association constant measurements.
A new method for the immobilization of alpha(1)-acid glycoprotein (AGP) in high-performance liquid chromatography (HPLC) columns was recently described for applications such as drug binding studies. Part of this earlier work used self-competition zonal elution studies to measure association equilibrium constants between immobilized AGP and R- or S-propranolol. It was later found that analysis o...
متن کاملChromatographic Studies of Protein-Based Chiral Separations
The development of separation methods for the analysis and resolution of chiral drugs and solutes has been an area of ongoing interest in pharmaceutical research. The use of proteins as chiral binding agents in high-performance liquid chromatography (HPLC) has been an approach that has received particular attention in such work. This report provides an overview of proteins that have been used a...
متن کاملGel Permeation Chromatography . I . A New Method for Molecular Weight Distribution of High Polymers
Polystyrene gels crosslinked in the presence of dilucnts have been iliatie in tinc-iiii.sli bead forni suitable for packing into chromatographic rolurnns. A series of narrow molecular weight range pol) mer fractions w&s eluted through such columns with aromatic and chlorinated solvents. EfHuent concentrations were detected and recorded by a continuous differential refractoineter. The fractions ...
متن کاملPreparation of protein-loaded PLGA-PVP blend nanoparticles by nanoprecipitation method: entrapment, Initial burst and drug release kinetic studies
Objective(s):Despite of wide range applications of polymeric nanoparticles in protein delivery, there are some problems for the field of protein entrapment, initial burst and controlled release profile. Materials and Methods: In this study, we investigated the influence of some changes in PLGA nanoparticles formulation to improve the initial and controlled release profile. Selected parameters ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Marmara Pharmaceutical Journal
سال: 2017
ISSN: 1309-0801
DOI: 10.12991/mpj.2017.17